1:
Applications of Next-Generation Sequencing to Horticultural Crops

Objective(s):
1. Illustrate uses of Next-Generation sequencing for gene discovery, expression profiling and transcriptome analysis 2. Discuss challenges of genome annotation using Next-Gen sequencing 3. Describe its use for enabling high throughput fingerprinting
New DNA sequencing instruments referred to as ‘next generation’ or ‘massively parallel’ sequencing platforms are becoming widely available and transforming genomic discovery in plants. They deliver an enormous amount of sequence data that require specialized and devoted computer infrastructure and bioinformatics. These technologies will impact many of the current uses of molecular tools in plant evolution, phylogenetics, gene discovery, fingerprinting, linkage mapping, and marker-assisted selection, among others. Use of these next-generation sequencing technologies has begun in vegetable and fruit crops.
In this workshop, we plan to offer an overview of next-generation sequencing technologies and their applications in horticultural crops.  Speakers selected for the colloquium will cover three main applications: Genome annotation; gene discovery and transcriptomics; and high throughput fingerprinting.
Monday, August 2, 2010: 2:00 PM
Desert Salon 7
Moderator:
Coordinator:
2:15 PM
Utilization of Next Generation Sequecing for Analyzing Transgenic Insertions in Plum: A Risk Assessment Study
Ann Callahan, USDA/ARS Appalachian Fruit Research Station; Chris Dardick, USDA-ARS, Appalachian Fruit Research Station; Ralph Scorza, USDA–ARS, Appalachian Fruit Research Station
2:35 PM
Tissue and Developmental Transcriptome Differences in Strawberry Revealed with Next-Generation Sequencing
Kevin Folta, PhD, Univ of Florida; Srikar Chamala, Univ of Florida; W. Bradley Babazuk, University of Florida; Asha Brunings, Univ of Florida; Maureen A. Clancy, Univ of Florida; Hernan Rosli, Instituto de Investigationes Biologicas; Marina Pombo, Instituto de Investigationes Biologicas; Marcos Civello
3:35 PM
SNP Mining in Blueberry Using Illumina GAII Sequencing
Nahla V. Bassil, Ph.D, USDA–ARS, NCGR; Barbara Gilmore, USDA–ARS, NCGR; Todd Mockler, PhD, Oregon State University; James W. Olmstead, University of Florida; Allan Brown, Plants for Human Health Institute, North Carolina Research Campus, North Carolina State University; Lisa J. Rowland, USDA-ARS, Genet. Imp. of Fruit & Vegetables Lab.
3:55 PM
Quantitative Differential Gene Expression with NGS to Uncover Regulators of Short-Day Induced Growth Cessation in Peach
Douglas Bielenberg, PhD, Clemson University; F.A. Feltus, Clemson University; S. Jimenez, Clemson University; C.E. Wells, Clemson University; Gregory L. Reighard, Clemson University
4:45 PM
Next-Generation Sequencing of the Tomato Transcriptome: a Resource for SNP Discovery, High Throughput Genotyping and Translational Research
David Francis, PhD, Ohio State University; Allen Van Deynze, Seed Biotechnology Center, University of California; John Hamilton, Michigan State University; Matthew Robbins, USDA ARS; Sung-Chur Sim, The Ohio State University; Walter DeJong; Robin Buell, Michigan State University; Robin Buell, Michigan State University
See more of: Colloquia