Search and Access Archived Conference Presentations

The 2009 ASHS Annual Conference

1548:
Full Length cDNA Sequence of Pear (Pyrus bretschneideri Rehd.) S29-RNase and S29-Allele Identification

Saturday, July 25, 2009
Illinois/Missouri/Meramec (Millennium Hotel St. Louis)
Lin Zhang, The key Lab. of Non-wood Forest Product of Forestry Ministry, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha,410004, China
Xiao-Feng Tan, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Hunan 410004, China
Donglin Zhang, Univ of Maine, Orono, ME
Yan Shen, Central South Univ of Forestry & Technology, Huann 410004, China
Yuan Deyi, Central South University of Forestry & Technology, Changsha, Huann, China
Chinese white pear (Pyrus bretschneideri Rehd.) belongs to Rosaceae that exhibits characteristic gametophytic self-incompatibility. This type of self-incompatibility is controlled by S-locus which carries a series of multi-allelic alleles encoding S-RNases. To elucidate the function of S-allele and the possible molecular mechanism of gametophytic self-incompatibility in Chinese white pear, full length cDNA encoding S29-RNase was cloned by rapid amplification of cDNA ends (RACE) approach from cultivars ‘Mili’ (S19S29) and ‘Zaomi’ (S19S29). The S29-RNase gene contained an open reading frame of 684 nucleotides encoding 228 amino acid residues. S29-RNase displayed typical sequence features of rosaceous S-RNases, i.e. five conserved regions (C1, C2, C3, RC4 and C5) and a hypervariable (HV) region. At the deduced amino acid level, S29-RNase showed 30% to 92% similarities with other rosaceous S-RNases. Phylogenetic analysis revealed that rosaceous S-RNases occurred before divergence of species, but after divergence of subfamilies Maloideae and Amygdaloideae. Genomic PCR amplification with primer combination FTQQYQ and anti-(I/T)IWPNV followed by digestion with the restriction enzyme AccII allowed effectively distinguishing S29-allele from other pear S-alleles.
See more of: Genetics/Germplasm/Plant Breeding: Biotechnology (Posters)
See more of: Oral and Poster Abstracts