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The 2009 ASHS Annual Conference

1919:
Analysis of Seed-Expressed Sequence Tags In Vernicia Fordii

Saturday, July 25, 2009
Illinois/Missouri/Meramec (Millennium Hotel St. Louis)
Lushan Xie, Central South Univ of Forestry & Technology, Huann 410004, China
Xiao-Feng Tan, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Hunan 410004, China
Lin Zhang, The key Lab. of Non-wood Forest Product of Forestry Ministry, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha,410004, China
Donglin Zhang, Univ of Maine, Orono, ME
Hongxu Long, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Hunan, 410004, China
Jiao Hu, Central South University of Forestry and Technology, Changsha, China
Tung-oil tree (Vernicia fordii) is one of the high-value industrial oil trees in China, whose seed has 52%-64% oil-yields content. The tung oil is widely used in painting and chemical industry. Its principal component is eleostearic acid (9,11,13-octadecatrienoic acid) that is a conjugated trienoic fatty acid. It is readily oxidized when exposed to air, which resulted in formation of the unique polymer. To isolate the genes related to tung oil synthesis and examine their expression patterns in V. fordii, we constructed a cDNA library using the developing seed tissues of V. fordii ‘duiniantong’ and picked out 3,107 clones randomly for sequencing on the 5'-end to construct the EST library. We then performed homology comparison with nucleic acid NR database. The results indicated that 2,205 cDNA sequences were highly homological to these sequences of other plant species in database of NCBI. A total of 482 different genes had been identified preliminarily and 342 clones possibly represented genes with unknown function. The sequences related to tung oil synthesis had delta 12 oleic acid desaturase (FAD2), omega-3 fatty acid desaturase, 3-ketoacyl-CoA thiolase, beta-ketoacyl-ACP synthase I, enoyl-acyl-carrier-protein reductase, esterase/lipase/thioesterase family protein, polyunsaturated fatty acid synthase subunit B, enoyl-CoA hydratase/isomerase family protein, and etc. Seed storage protein had caleosin, oleosin, legumin, albumin, and etc. There are lots of ribosomal RNA and pollen allergen genes. Many sequences corresponded to transfer protein, gene expression, development regulation, resistance, growth substances and embryogenesis, and etc., while the sequences of 338 genes related to universal substances biochemical metabolism were expressed only once. The numbers and trends of expressed genes were at the phase of V. fordii seeds near to late embryogenesis. Establishing the EST library of V. fordii and researching the genes related to the tung oil synthesis have great significance to producing high-quality tung oil.
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