3176:
Serology, Transmission and Protein Analysis for Diagnosis of Citrus Tristeza Closterovirus in the Punjab-Pakistan

Thursday, August 5, 2010: 10:15 AM
Springs K & L
Yasir Iftikhar, PhD, Plant, Pathology , University College of Agriculture, University of Sargodha, Sargodha, Pakistan
Sardar M. Mughal, PhD, Plant, Pathology , Department of Plant Pathology, PMAS Arid Agriculture University, Department of Plant Pathology, PMAS Arid Agriculture University, Rawalpindi, Rawalpindi, Pakistan
Muhammad Mumtaz Khan , Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan
Muhammad Aslam Khan, PhD, Plant, Pathology , Department of Plant Pathology, University of Agriculture, Faisalabad, Department of Plant Pathology, University of Agriculture, Faisalabad. Pakistan, Faisalabad, Pakistan
Zahoor Hussain , Department of Horticulture, University College of Agriculture, University of Sargodha,, Sargdoah, Pakistan
Raheel Anwar , Institute of Horticultural Sciences, Purdue University, West Lafayette, IN
Fifty samples of each of sweet orange (Malta and Mosambi), kinnow mandarin and grapefruit collected from five locations of the Punjab (2007-2008) were analysed through DAS-ELISA for the diagnosis of CTV, using polyclonal antibodies. On the basis of ELISA positive reaction, incidence of CTV in the locations ranged from 20-45% with the mean of 32% infection. The high number of ELISA positive samples were found at Bhalwal with mean OD260 nm (0.46), followed by samples at Sahiwal (0.29), Faislabad (0.24), Toba Tek Singh and Sargodha (0.17). Polypeptide profile was analysed through SDS-PAGE for the leaf and bark protein of citrus samples but no significant changes were found except slight alteration in the electrophoresed protein in sweet orange. Symptoms of mild leaf flecking were produced in acid lime after mechanical inoculation of sap from infected sweet orange, but aphid transmission using Aphis gossypii and whitefly was not successful. Therefore, insect transmission of CTV constitutes subject of future investigation.
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