Transformation of Grapevine

Agrobacterium-mediated transformation of antifreeze protein gene in ‘Kyoho’ and ‘Cabernet Sauvignon’ Grapevine.  

Hye-Young Shin, Gi-Hoon Kim, Cheol Choi*

Department of Horticulture Graduate School, Kyungpook National University, Daegu 702-701, Korea

Abstract

The optimum condition for shoot regeneration from leaf explants of grapevine cultivar ‘Kyoho‘ and ‘Cabernet Sauvignon’ were investigated and it was originating from in vitro cultures were transformed. It was transformation with Agrobacterium tumefaciens strain LBA4404 harbouring a binary vector pBI121 which contained a neomycin phosphotransferase II (NPTII) gene and AFP gene encoding antifreeze protein from carrot. The explants were co-cultivated for 3days with recombinant Agraobacteria and then selection on Murashige and Skoog (MS) basal medium supplemented with concentrations of 5mg/L Thidiazuron (TDZ), 0.1mg/L Indole-3-Butyric Acid (IBA), 100mg/L kanamycin and 500mg/L carbenicilln. Successful regeneration of transgenics were obtained. PCR analysis were verifed the integration of the antifreeze protein(AFP) and neomycin phosphotransferase II (NPTII) genes into genomic DNA.

* corresponding author : cc31@knu.ar.kr