Clonal Propagation of Guava On Nodal Explants of Mature Trees

A regeneration system using the MS medium with thidiazuron (TDZ) and naphthaleneacetic acid (NAA) was developed for guava (Psidium guajava L.) from mature tree cultivars. To determine the best means for proliferation we tested different disinfection methods and plant growth regulators on in vitro culture establishment. The most effective method involved treating explants in a 15% bleach solution for 20 mins then culturing them in the medium with 250mg/L PVP. This method maximized the percentage of breaking new buds (53.3%) with the minimum browning rate (18.3%) for the explants.  In terms of levels of TDZ and NAA, the best regeneration for guava (80%) was observed on medium with 1mg/L BA, 1mg/L Kinetin and 0.1mg/L NAA, producing the highest mean number of shoots (2.4). Shoots were then rooted (65%) when dipped in 1mg/ml IBA for 1 min and rooted plantlets survived after acclimatization to the greenhouse.