Construction of the Genetic Linkage Maps for the Diploid and Tetraploid Rose Using Simple Sequence Repeat (SSR) and AFLP Markers

Construction of the Genetic Linkage Maps for the Diploid and Tetraploid Rose Using Simple Sequence Repeat (SSR) and AFLP Markers

Ching-Jung Tsai, Tzili Pleban, Oron Gar, Dani Zamir and David H. Byrne

Department of Horticultural Sciences, Texas A&M University, College Station, TX 77843-2133 USA
The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew university of Jerusalem, Rehovot, 76100 Israel

Low density genetic linkage maps for both the OBxWOB26 (Old Blush x (Basye’s Thornless x Old Blush) diploid backcross population and the FCxGG (Fragrant Cloud x Golden Gate) tetraploid F1 population were constructed with 68 and 57 labeled SSR primers, respectively. These were multiplexed using fluorescently labeled (HEX and FAM) primers that after PCR were run on a capillary sequencer (ABI 3100). This was done as a first step to develop a consensus rose map with European collaborators. Among 68 SSR markers, 53 primers were uni-parental markers, which are heterozygous in either the male or female parent, whereas 15 primers were bi-parental markers in which both parents were heterozygous. Moreover, 5 out of the 68 SSR markers, RhJ404, H9_B01, RW11E5, RW8B8 and RhE3 were mapped to two or more loci each. Thus a total of 75 loci were mapped using JoinMap 4.0 to create a diploid map with seven integrated linkage groups covering a length of 413 cM with an average chromosome size of 59 cM. In the integrated diploid map, the morphological traits, recurrent bloom (R/r, seasonal/recurrent) and flower type (Blfo) were mapped near the markers Rh58 and Rh50 at a distance of 0.1 and 0.8 cM, respectively.  Flower type (Blfo) was previously shown to be closely linked to the marker Rh 50 (Yan, 2005) in the 93/1-119 map. However, the phenotypic trait, presence of stem prickles although mapped to the same chromosome as recurrent bloom and flower type, was not located near any markers. Fifty seven SSR and seventy nine AFLP markers have been used to develop the linkage map for the tetraploid rose. These primers were assigned to 16 linkage groups and cover approximately 1232 cM of the genome with an average chromosome length of 88cM. The two integrated diploid and tetraploid maps will be combined to a unified map using JoinMap 4.0 in near future.