The 2010 ASHS Annual Conference

Effect of basic medium and plant growth regulators on in vitro multiplication of Phaius tancarvilleae (Banks ex L^, Heritier) Blume was studied. There were 3 experiments. First experiment: effect of basic medium on protocorm like body (PLB) induction was investigated. Buds were cultured on 5 basic media which were Vacin and Went (1949), Thomale GD (1954), Murashige and Skoog (1962), Knudson C (1946) and White (1963). Each medium was added 0.1 mg/l NAA and 0.5 mg/l kinetin. Second experiment: The best liquid basic medium (from first experiment) containing a combination of 0, 0.002, 0.004, 0.006, 0.008 and 0.010 mg/l Triacontanol and 0, 0.1, 0.5 and 1.0 mg/l BA was studied. Third experiment: the medium supplemented with the same concentration of triacontanol and BA as second experiment except the medium of this experiment was solid. After 16 weeks of incubation, the maximum number of PLB (3.25), the highest percentage of explants produced PLB (75) and the biggest width of explants (1.29 cm.) were obtained from Vacin and Went medium. In second experiment, after 12 weeks of incubation, the maximum number of PLB (6.75), the highest percentage of explants produced PLB (91.67), the biggest width of explants (1.12 cm.) and the heaviest fresh weight of explants (0.45 g) achieved from Vacin and Went  liquid medium containing 0.004 mg/l Triacontanol and 0.1 mg/l BA. In third experiment, after 8 weeks of incubation, the maximum number of shoot (7.38 shoot), the highest number of PLB (1.50), the biggest width of explants (1.90 cm.) the heaviest fresh weight of explants (1.8 g) and the maximum number of root (4.37) also obtained from Vacin and Went solid medium supplemented with 0.004 mg/l triacontanol and 0.1 mg/l BA.