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The 2010 ASHS Annual Conference

4219:
Effect of BA and NAA On in Vitro Multiplication of Butterwort (Pinguicula gigantea)

Monday, August 2, 2010
Springs F & G
Kanjana Saetiew, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok, Thailand
Vasan Sang-In, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok, Thailand
Sumay Arunyanart, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok, Thailand
Effect of BA and NAA on in vitro multiplication of Butterwort (Pinguicula gigantea) was studied. Leaves were cultured on MS (Murashige and Skoog, 1962) medium supplemented with 0.2, 2.0, 10.0 and 20.0 mg/l BA and 0.1, 1.0, 5.0 and 10.0 mg/l NAA, 3% sucrose, 0.8% agar. The pH of medium was adjusted to 5.7. It was found that the maximum number of shoots (21.75 shoots) were obtained form medium with 2.0 mg/l BA and 0.1 mg/l NAA when cultured on media for 8 weeks. The heaviest weight of explants (0.57 g.) achieved from medium with 2.0 mg/l BA and 5.0 mg/l NAA. The MS medium with 0.2 mg/l BA and 5.0 mg/l NAA gave the average of longest shoots (0.83 cm.)
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