The 2010 ASHS Annual Conference

Objective(s):
1. Illustrate uses of Next-Generation sequencing for gene discovery, expression profiling and transcriptome analysis 2. Discuss challenges of genome annotation using Next-Gen sequencing 3. Describe its use for enabling high throughput fingerprinting

New DNA sequencing instruments referred to as ‘next generation’ or ‘massively parallel’ sequencing platforms are becoming widely available and transforming genomic discovery in plants. They deliver an enormous amount of sequence data that require specialized and devoted computer infrastructure and bioinformatics. These technologies will impact many of the current uses of molecular tools in plant evolution, phylogenetics, gene discovery, fingerprinting, linkage mapping, and marker-assisted selection, among others. Use of these next-generation sequencing technologies has begun in vegetable and fruit crops.
In this workshop, we plan to offer an overview of next-generation sequencing technologies and their applications in horticultural crops.  Speakers selected for the colloquium will cover three main applications: Genome annotation; gene discovery and transcriptomics; and high throughput fingerprinting.