Assessment of Genetic Diversity In Leafy Radish with Different Heat Tolerant Ability by ISSR Maker and Vegetative Traits

    Leafy radish (Raphanus sativus L. var. oleiformis Pers.) is newly fresh vegetable in Taiwan and breeding for heat-tolerant variety is a commercial interest. However, the diversification of cultivated leafy radish and its relationship with heat tolerant has not been well understood. Thus, in our study, the genetic diversity of 21 leafy radish cultivars/lines with different heat tolerant abilities was assessed by inter-simple sequence repeat (ISSR) maker and vegetative traits. Detected by 21 primers, 293 bands were amplified and the proportion of polymorphic bands was 76.45%. The results of UPGMA and PCA analysis based 28 vegetative traits were similar to ISSR maker. It showed that the genetic diversity of tested leafy radish cultivars/lines was highly associated with their own reigns and main characteristics such as leaf shape, trichome number, the presence of anthocyanin in petiole, but not accordance with heat tolerant ability.