The 2011 ASHS Annual Conference

Flax (Linum usitatissimum L.) seeds were germinated on agar with 1% sucrose.  When hypocotyls reached 2 cm in length, they were cut into 0.75 cm pieces.  Eight hypocotyls were placed per petri  plate containing MS media at pH 5.8, 3% sucrose, and 0.8% agar.  A total of 10 plates were used per treatment  and experiment was repeated three times.  Filter sterilized methyl jasmonate was added at 0.050 µM, 1.0 µM, 5.0 µM, 10.0 µM concentrations.   Filter sterilized glutathione was added at 100 µM, 200 µM, and 300 µM.  Methyl jasmonate effects were concentration dependent, and inhibited callus formation.  Glutathione effects were also concentration dependent, with significant increase of root growth in callus at 300 µM.  Shoot growth was inversely related to the glutathione concentration.