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The Effect of Temperature and Sugar Content on Pollen Viability in Chinese Cabbage, Poster Board #142

Wednesday, August 1, 2012
Grand Ballroom
Jumsoon Kang , Department of Horticultural Bioscience, Pusan National University, Miryang, South Korea
Beunggu Son , Department of Horticultural Bioscience, Pusan National University, Miryang, South Korea
Youngwhan Choi , Department of Horticultural Bioscience, Pusan National University, Miryang, South Korea
Yongjae Lee , Department of Horticultural Bioscience, Pusan National University, Miryang, South Korea
Jeongmin Suh , Department of Bioenvironmental Energy, Pusan National University, Miryang, South Korea
Younghoon Park , Department of Horticultural Bioscience, Pusan National University, Miryang, South Korea
Pollen grains of Chinese cabbage germinated 90% in 6 hrs after incubation, regardless of temperature. Both cultivars showed the highest pollen germination rate and pollen tube length at temperature ranges of 25–30 °C. Higher temperature increased speed of pollen tube growth and it was 180 µ/hr at 30 °C. Pollen tube growth was retarded by lower temperature. After 24 hrs incubation, the length of pollen tube was 2,500 µ at 15 °C, just below 50% of tube length at 20 °C. Pollen grain viability was higher when germination media contained 10% to 20% sucrose. Pollen grain and pollen tube rupture during germination were observed in media with below 10% sucrose. After pollen incubation at 25 °C for 12 hrs, 84% of pollen germinated at a sucrose medium, but 60% and 42% at glucose and fructose media, respectively. Optimum pH in germination media was at the range of 6.0–7.0. Light condition during germination appeared to retard pollen viability.
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