Tuesday, July 31, 2012
Grand Ballroom
Tomato is one of annual plant and cultivated mostly in greenhouse. Most of tomato plant has been discarded as a waste after fruit harvesting although tomato plants, not fruit have many physiological functions like antioxidant activity and whitening effects. Therefore, in this study, various parts of tomato plants (leave, stem, root, or fruit) were evaluated their antioxidant and whitening effect for cosmetic purpose. Tomato samples were freeze-dried, extracted with 80% methanol. Antioxidant activities of tomato plants were determined by DPPH radical scavenging and ABTS radical scavenging. Whitening effect of tomato samples were studied by tyrosinase inhibition or melanin synthesis extent on B16 melanoma cell line. Tomato leave extract showed the strongest antioxidant activity with 31% DPPH scavenging, 88.7% ABTS scavenging among samples. For whitening effect of tomato extracts, leave extracts showed a 1.5–2 times higher tyrosinase inhibition than those of stem or fruit. Leave and root extracts showed above 70% inhibition of melanin synthesis on B16 melanoma cell. In conclusion, tomato leaves showed the highest antioxidant activity and whitening effect among samples. Therefore, these results indicated that tomato leave could be applicable for functional cosmetic material in industry field instead of throwing away.