The 2012 ASHS Annual Conference

Despite many studies with acerola (Malpighia emarginata), showing the great benefits of this fruit to health mainly as an extraordinary vitamin C source, the research on antioxidants present in processing waste, its association with bioactive compounds and their role in food quality and health of the consumer is an emerging research area that has been little explored. This study aimed to evaluate the waste generated by the processing industry of juice as a natural source of antioxidant dietary fiber (ADF). The waste from the juice processing, pulping and refining steps, were provided by a industry and transported to Embrapa Tropical Agroindustry, Fortaleza, CE, Brazil. The samples divided into three replications were lyophilized and then, ground and stored at -20 °C. The determination of FD followed the method of indigestible fraction based on physiological conditions that mimics the human digestive process and involves of a series of enzymatic treatments (pepsin, pancreatin and α-amylase) which resulted in a residue (insoluble DF - IDF) and supernatant. The latter was subjected to incubation with amyloglucosidase followed by a dialysis system, resulting in soluble DF (SDF). The non-starch polysaccharides (NSPs Total) expressed by the sum of the results of Dinitrosalicylate, Antrona, and Uronic acids methods were determined in the SDF and IDF, and Klason lignin (LK), gravimetric weight, protein resistant (PR) and ash analyzed just in IDF. The total antioxidant capacity (TAC) associated with SFD (total extractable polyphenols - TEP) and FDI (TEP, hydrolysable tannins – HT, and condensed tannins CT) was determined by DPPH•. The results expressed in % of dry matter, in the pulping and refining wastes, were: FD Total - 83.50% and 77.81%; IDF - 92.35% and 84.96%; KL - 48.85% and 32.37%; NSPs Total - 29.20% and 33.81%, PR - 1.08% and 5.88%, Ash - 2.67% and 3.85%, TEP / FDS - 1.04% and 1.5%, TEP / FDI - 0.66% and 0.40%; HT / FDI - 0.58% and 0.63%, TC / IDF - 0.31% and 0.24%, TAC (TEP / FDS) - 68.32 and 77.62 g / g DPPH •; TAC (TEP / IDF) - 90.66 and 52.91 g / g DPPH •; TAC (TH) - 38.7 and 16.94 g / g DPPH •. The waste of juice processing present a high content of Total FD (> 70%) associated with a high CAT, which could be considered as a high functional raw material and consequently can add value to several byproducts.