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The 2012 ASHS Annual Conference

9931:
Simple Sequence Repeat (SSR) Markers Associated with White Flower in Peach [Prunus persica (L.) Batsch] Identified using Bulked Segregant Analysis (BSA)

Thursday, August 2, 2012
Grand Ballroom
Dario J. Chavez, Horticultural Sciences Department, University of Florida, Gainesville, FL
Thomas G. Beckman, Ph.D., USDA–ARS, Byron, GA
José Chaparro, University of Florida, Gainesville, FL
White flower color is controlled by a recessive allele w in peach. To map the W locus, open pollinated seed were collected from two different F1 hybrids. The first hybrid was an F1 between ‘White English’ ww (white flower) and ‘UFSun’ WW (pink flower). The second a hybrid was an F1 between ‘Brooks’ ww (white flower) and ‘UFBeauty’ WW (pink flower).  Open pollinated seed were collected from both F1 hybrids to produce a segregating F2 population. ‘Brooks’ × ‘UFBeauty’ and ‘White English’ x ‘UFSun’ F2 populations consisted of 37 and 110 individuals, respectively.  The F2 populations did not deviate significantly from a 3:1 pink to white phenotypic segregation ratio (c2 ≤ 0.01). White flower phenotypes were identified to be homozygous recessive, as previously described. Bulked samples of white and pink flower phenotypes from ‘White English’ × ‘UFSun’ F2 population were used to identify the genomic region linked with white flower ww locus using a total of 41 simple sequence repeat (SSR) markers. A linkage map was created with the SSR markers closely linked with white flower ww in peach and the locus placed on the Prunus reference genome map.
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