The 2012 ASHS Annual Conference
9931:
Simple Sequence Repeat (SSR) Markers Associated with White Flower in Peach [Prunus persica (L.) Batsch] Identified using Bulked Segregant Analysis (BSA)
9931:
Simple Sequence Repeat (SSR) Markers Associated with White Flower in Peach [Prunus persica (L.) Batsch] Identified using Bulked Segregant Analysis (BSA)
Thursday, August 2, 2012
Grand Ballroom
White flower color is controlled by a recessive allele w in peach. To map the W locus, open pollinated seed were collected from two different F1 hybrids. The first hybrid was an F1 between ‘White English’ ww (white flower) and ‘UFSun’ WW (pink flower). The second a hybrid was an F1 between ‘Brooks’ ww (white flower) and ‘UFBeauty’ WW (pink flower). Open pollinated seed were collected from both F1 hybrids to produce a segregating F2 population. ‘Brooks’ × ‘UFBeauty’ and ‘White English’ x ‘UFSun’ F2 populations consisted of 37 and 110 individuals, respectively. The F2 populations did not deviate significantly from a 3:1 pink to white phenotypic segregation ratio (c2 ≤ 0.01). White flower phenotypes were identified to be homozygous recessive, as previously described. Bulked samples of white and pink flower phenotypes from ‘White English’ × ‘UFSun’ F2 population were used to identify the genomic region linked with white flower ww locus using a total of 41 simple sequence repeat (SSR) markers. A linkage map was created with the SSR markers closely linked with white flower ww in peach and the locus placed on the Prunus reference genome map.