Assessment of Genetic Diversity of Commiphora wightii (Guggal) Germplasm in Rajasthan using RAPD and ISSR Markers

Monday, July 22, 2013
Desert Ballroom: Salons 7-8 (Desert Springs J.W Marriott Resort )
Priyanka Vyas , Government College, Ajmer, Jodhpur, India
Ramesh Joshi , Government College, Ajmer, Ajmer, India
M.L. Agarwal , Government College, Ajmer, Ajmer, India
Dilip Nandwani , Agricultural Experiment Station, University of the Virgin Islands, Kingshill, US Virgin Islands
The aim of the present study is to evaluate genetic diversity within and among populations of Commiphora wightii (Guggal) in different regions of Rajasthan using RAPD and ISSRs markers. An extensive survey was conducted to identify the plant gender in each population. All the plants from Mangaliyawas, Pushkar, and Ajmer were found to be hermaphrodite.  Plant materials were collected from different locations in Rajasthan namely Ajmer, Pushkar, Mangaliyawas, Jodhpur, Udaipur, and other locations where Commiphora wightii grows in natural conditions. Genomic DNA was isolated from 3 g leaf tissues from all accessions with the modified CTAB method. The DNA was treated with RNase for eradication of RNA. The DNA concentration was estimated using the spectrophotometric method. Absorbance of the solution was measured at wavelengths 260 nm and 280 nm. The DNA was diluted to 30 ng/µl for RAPD analysis. Sixty 10-base primers were used for polymerase chain reaction for screening of genetic diversity to ascertain their potential of clear amplification in polymorphism and reproducibility. The RAPD and ISSR profiles were produced through PCR amplification and polymorphism was recorded among the population of C. wightii in Rajasthan. The results of the research will help in determination of gender in early stages of development and establishment of the relationship of genetic variability with geographical distribution in Rajasthan and also, offer genetic resource information for future meaningful implementation of conservation programs applicable for C. wightii.