Transcriptome Analysis of Vernicia fordii Seed in Three Development Stages

Thursday, July 25, 2013: 10:45 AM
Desert Salon 9-10 (Desert Springs J.W Marriott Resort )
Xiao-Feng Tan , Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Hunan 410004, China
Hongxu Long , Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Hunan, 410004, China
Guixiong Jiang , Guangxi Academy of Forestry, Nanning, 530002, China
Lin Zhang , Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha,410004, China
Donglin Zhang , University of Georgia, Athens, GA
Fangfang Yan , Central South University of Forestry & Technology, Changsha,410004, China
To investigate the profile of gene expression in Vernicia fordii and elucidate its functional gene, Illumina HISEQ 2000 sequencer was used to produce a substantial expressed sequence tags dataset from the seed of 'Pu tao tong' in three development stages, fruit-inflating stage (sample 1, 60 days after flowering), lipid synthesis early stage (sample 2, 120 days after flowering), and lipid synthesis peak stage (sample 3, 150 days after flowering).  Totals of 61,001 unigenes, 54,679 unigenes, and 44,495 unigenes were obtained from 3 samples, respectively. A total of 58,439 unigenes with average length of 889nt and 41,059 unigenes with protein function annotations were generated. The Nr annotation of tung tree unigenes showed that the biggest number (64.04%) among all unigienes could find homologous genes of Ricinus communis, followed by Populus trichocarpa (17.4%), Vitis vinifera (8.98%), Glycine max (1.81%), Jatropha curcas (1.08%), and others (6.69%).  All unigenes can be classified into 25 functional-categories, of which 4,865 unigenes were related to the general function prediction only, and then 2,403 unigenes were related to transcription, 639 unigenes were related to lipid transport and metabolism. All unigenes were queried against the KEGG pathway database, and 22,134 unigenes were given the pathway annotations and related to 128 pathways, including metabolism, biosynthesis of secondary metabolites, fatty acid biosynthesis, biosynthesis of unsaturated fatty acids and so on.  Analysis of different expression genes showed that the up-regulation and down-regulation genes have significant difference in 3 development stages of Vernicia fordii seed. Between Sample 1 and Sample 2, 9,057 up-regulated unigenes and 4,531 down-regulated unigenes were revealed. Between Sample 1 and Sample 3 there were 21,972 up-regulated unigenes and 2,938 down-regulated unigenes, and 1,468 up-regulated unigenes and 14,069 down-regulated unigenes between Sample2 and Sample 3. Data presented in the study will constitute an important resource for the data mining of important genes and the research of regulation and expression mechanism. This study also provides scientific evidence for breeding of Vernicia fordii.
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