Proteomic Analysis of Gibberellins Increased Fruit Sink Strength in Pyrus pyrifolia

Wednesday, July 24, 2013: 8:30 AM
Springs Salon A/B (Desert Springs J.W Marriott Resort )
Jiefa Li , Shanghai Jiao Tong University, Shanghai, China
Shiping Wang , Dept of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China
Wenping Xu , School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China
Bo Wang , Shanghai Jiao Tong University, Shanghai, China
Xiuming Yu , Shanghai Jiao Tong University, Shanghai, China
Lei Wang , Shanghai Jiao Tong University, Shanghai, China
Yusui Lou , Shanghai Jiao Tong University, Shanghai, China
Qi Yang , Shanghai Jiao Tong University, Shanghai, China
Caixi Zhang , School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China
Our previous work demonstrated that exogenous gibberellins (GAs) applications during rapid fruit growth significantly increases sink demand and results in a larger fruit in Pyrus pyrifolia. To better understand the sizing effect of GA on pear fruit, the pear fruit (Pyrus pyrifoliaNakai cv. Cuiguan) was treated with GA and differential protein expression were determined by proteome analysis. Differential in-gel electrophoresis (DIGE), Matrix-assisted laser-desorption/ionization Time of flight/Time of flight (MALDI-TOF/TOF) were performed. In this study, 66 proteins were successfully identified and assigned to 8 functional groups. Among the 66 identified proteins, 9 protein spots were related to stress response/defense, 7 were associated with expression regulation and epigenetic, 4 with cell structure, 23 with photosynthetic and energy, 3 with signal transduction, 4 with protein synthesis/fate; 11 with transport, and 5 with unknown function. According to the expression differences of the proteins, GAs had a significant influence on the abundance of differently-expressed proteins between core and sarcocarp. The present data will help to shed light on pear fruit ripening process.
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