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2013 ASHS Annual Conference

14274:
Anti-inflammatory Activity of Potentilla kleiniana Extracts

Wednesday, July 24, 2013
Desert Ballroom: Salons 7-8 (Desert Springs J.W Marriott Resort )
Jung-Hwan Nam, Highland Agricultre Research Center, National Institute of Crop Science, RDA, Highland Agriculture Research Center, NICS, RDA, Pyeongchang, South Korea
O.S. Hur, Rural Development Administration, NAAS, Suwon, South Korea
Su-Young Hong, Rural Development Administration, National Institute of Crop Science, Pyeongchang, South Korea
Su Jeong Kim, Highland Agriculture Research Center, Highland Agriculture Research Center, NICS, RDA, Pyeongchang, South Korea
Hee-Jhun Park, Pharmacydoctor, Pharmaceutical Engineering, Sangji University, College of Health Sciences, Wonju, South Korea
Kyung-Tea Lee, Pharmacydoctor, Pharmaceutical, Kyung-Hee University, College of Pharmacy, Seoul, South Korea
Oh-Keun Kwon, Rural Development Administration, National Institute of Crop Science, Pyeongchang, South Korea
Kwang-Soo Cho, Rural Development Administration, National Institute of Crop Science, Pyeongchang, South Korea
Hwang-Bae Sohn, Rural Development Administration, National Institute of Crop Science, Pyeongchang, South Korea
Ki Sun Kim, Seoul National University, Seoul 151-921, South Korea
Potentilla kleiniana (PK) is a member of the Rosaceae family that is native to China, Japan, Korea, India, and Malaysia. It produces one or more erect stems from a branching caudex and system of rhizomes. It grows 20 to 60 cm tall, and is slightly hairy to nearly hairless. The leaves are ternate, divided into three leaflets. The basal leaves are largest, borne on long petioles. Each has oval leaflets up to 3 cm long that are deeply cut into blunt teeth. Smaller leaves occur higher on the stem. The inflorescence is a cyme of one or more flowers. The flower has usually five yellow petals up to a cm long on a calyx of pointed sepals and narrower pointed bractlets. Potentilla kleiniana is often used in Korean traditional systems of medicine as a remedy for hemostasis, dysentery cough, pertussis, sore throat, external bleeding, and neutralizes snake venom. Generally drugs that are used for arthritis have antinociceptive and anti-inflammatory properties. However, validity of the anti-inflammatory activity has not been scientifically investigated so far. Therefore, the aim of this study was to investigate the anti-inflammatory potential of PK using the ethanolic extract of PK and its sub-fractions. To evaluate the anti-inflammatory effects of PK, we examined the inflammatory mediators such as nitric oxide(NO) and prostaglandin E2 (PGE2) on RAW 264.7 cells. Our results indicated that ethanolic extract significantly inhibited the LPS-induced NO, and PGE2 production in RAW 264.7 cells. The ethanolic extract inhibitory activity for NO and PGE2 tests with IC50 values showed in 49.57 μg/ml. This result revealed that ethanolic extract of PK is expected to be good candidate for development into source of anti-inflammatory agent.