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2013 ASHS Annual Conference

14554:
Cloning and Characterization of a Stearoyl–Acyl Carrier Protein Desaturase Gene from Tung Tree (Vernicia fordii)

Monday, July 22, 2013
Desert Ballroom: Salons 7-8 (Desert Springs J.W Marriott Resort )
Lin Zhang, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha,410004, China
Min Liu, Central South University of Forestry & Technology, Changsha,410004, China
Xiao-Feng Tan, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Hunan 410004, China
Hongxu Long, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Hunan, 410004, China
Donglin Zhang, University of Georgia, Athens, GA
Qirui Wang, Henan Academy of Forestry, Zhengzhou, 450008, China
Zhibo Song, Central South University of Forestry & Technology, Changsha,410004, China
Baoguang Jia, Central South University of Forestry & Technology, Changsha,410004, China
The stearoyl–acyl carrier protein desaturase (SAD), a key enzyme, determines the ratio of saturated to unsaturated fatty acids in higher plants. Using the methods of reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE), a full-length cDNA encoding SAD was obtained from developing seeds of Tung Tree (Vernicia fordii) that was named VfSAD and deposited under GenBank (Accession no. GU363502). The VfSAD contained an open reading frame of 1179 nucleotides encoding 392 amino acid residues. At the deduced amino acid level, the VfSAD showed 76% to 96% similarities with other other reported SADs. The predicted isoelectric point (pI) and molecular weight (Mw) of VfSAD is 5.99, 45217.7 Da, respectively. The VfSAD is predicted to be a kind of hydrophilic and non-secreted proteins. The predicted VfSAD contained several functional domains including N-glycosylation sites, cAMP and cGMP dependent protein kinase phosphorylation sites, and a FA_desaturase_2 motif. Quantitative RT-PCR analysis revealed that the VfSAD was expressed at all of the stages of V. fordii seeds, but displayed an irregular expression profile. These results would provide a base for understanding the mechanism of fatty acid composition and modifying the fatty acid composition in V. fordii.
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