2013 ASHS Annual Conference

To obtain virus-free bulbs in hybrid lily progenies, scale propagation of apical meristem culture was performed in vitro for each cross combination. Meristems sized 0.5 mm or less were isolated from bulblets and cultured in MS media containing 0.5 mg/L IAA and the following growth regulators: 2, 3, or 5 mg/L BA and 0.2, 0.3, or 0.5 mg/L TDZ. We observed that 0.2 mg/L TDZ was effective in increasing the number of shoots to 10.6, but shoot length trended toward decrease with the addition of growth regulators, regardless of type or concentration. Fresh weight was greater with 5.0 mg/L BA or 0.3mg/L TDZ treatment. For the FO and FLA progeny, treatment with BA and TDZ led to similar effects on the number and length of shoots and fresh weight, according to the type and concentration of the growth regulator, although the degree of changes was different. To grow proliferate bulblets from seedlings obtained through apical meristem culture, each hybrid progeny bulblets was cultivated on MS medium with 5.0 mg/L BA and 0.5 mg/L NAA. Medium containing 5.0 mg/L BA and 0.5 mg/L NAA was effective for shoot growth in the FLA progeny.