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2013 ASHS Annual Conference

14758:
The Effect of Growth Regulator Addition on Bulb Cultivation in Interspecific Hybrid Lilies

Monday, July 22, 2013
Desert Ballroom: Salons 7-8 (Desert Springs J.W Marriott Resort )
SeungDeok Kim, Chungcheongbuk-do Agricultural Research & Extention Services, Cheongwon-gun, Chungbuk, South Korea
JuHyung Kim, Chungcheongbuk-do Agricultural Research & Extention Services, Cheongwon-gun, Chungbuk, South Korea
JongWon Lee, Chungcheongbuk-do Agricultural Research & Extention Services, Cheongwon-gun, Chungbuk, South Korea
KiYeol Lee, Horticultural Research Division, Chungcheongbuk-do Agricultural Research & Extention Services, Cheongwon-gun, Chungbuk, South Korea
Taejung Kim, Horticultural Department, Chungcheongbuk-do Agricultural Research & Extention Services, Cheongwon-gun, Chungbuk, South Korea
Kee Yoeup Paek, Chungbuk National University, Cheongju 360-763, South Korea
To obtain virus-free bulbs in hybrid lily progenies, scale propagation of apical meristem culture was performed in vitro for each cross combination. Meristems sized 0.5 mm or less were isolated from bulblets and cultured in MS media containing 0.5 mg/L IAA and the following growth regulators: 2, 3, or 5 mg/L BA and 0.2, 0.3, or 0.5 mg/L TDZ. We observed that 0.2 mg/L TDZ was effective in increasing the number of shoots to 10.6, but shoot length trended toward decrease with the addition of growth regulators, regardless of type or concentration. Fresh weight was greater with 5.0 mg/L BA or 0.3mg/L TDZ treatment. For the FO and FLA progeny, treatment with BA and TDZ led to similar effects on the number and length of shoots and fresh weight, according to the type and concentration of the growth regulator, although the degree of changes was different. To grow proliferate bulblets from seedlings obtained through apical meristem culture, each hybrid progeny bulblets was cultivated on MS medium with 5.0 mg/L BA and 0.5 mg/L NAA. Medium containing 5.0 mg/L BA and 0.5 mg/L NAA was effective for shoot growth in the FLA progeny.
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