2013 ASHS Annual Conference

Many isothiocyanates (ITCs), are a mainly hydrolysis product in glucosinolates (GSLs), have been demonstrated the noteworthy overcoming impact against the survival and proliferation of cancer cells and their modulation of apoptosis and cell cycle progression by numerous molecular basis studies (Zang et al., 2006) , such as sulforaphane (SFA) isolated from broccoli seed and sprouts. By the way, sulforaphene (SFE), is a major ITCs in radish seed, have been reported the potency of biological activity, a little bit recently. On the other hands, while much researches were known that SFA in broccoli has the excellent anticancer effects such as induction of apoptosis and detoxification enzymes in vitro and in vivo (Fahey et al., 2002), SFE in radish was hardly the biological study in spite of their similar chemical structure in comparison with SFA. In the present study, I demonstrated the broadly biological activity of SFE against cancer cells, Helicobacter pylori (H. pylori) and multi-drug resistance pathogens. In 4 cancer cells isolated from each four organisms were notably inhibited the proliferation treated with purified SFE (IC₅₀ = 10.0–23 µg/mL). I also characterized that SFE modulated an induction of apoptosis pathway against A549 cancer cell through the proteins expressions related with apoptosis pathway. In addition, the highly bacteriocidal potency (MIC₉₀ = 0.6–5.0 µg/mL) of SFE was exhibited against H. pylori, particularly antibiotic resistant strain (212 strain, MIC₉₀ = 0.6 µg/mL). MRSA (Methicillin-resistant staphylococcus aureus), is known as super bacteria, also were inhibited by SFE (MIC₉₀ = 10–20 µg/mL), whereas the MIC ₉₀ value of MSSA (Methicillin-susceptible staphylococcus aureus) by SFE had little significant. These results suggested that the antibiotic potency of SFE in radish seeds would be associated with the potency in a broad range of cancer cells and antibiotic resistant pathogens.