2013 ASHS Annual Conference
15980:
S2 Progeny of Japanese Pear (Pyrus pyrifolia Nakai) 'Osa Nijisseiki' Having Self-compatibility and Their Homozygosity Estimated By SSR Analysis
15980:
S2 Progeny of Japanese Pear (Pyrus pyrifolia Nakai) 'Osa Nijisseiki' Having Self-compatibility and Their Homozygosity Estimated By SSR Analysis
Wednesday, July 24, 2013
Desert Ballroom: Salons 7-8 (Desert Springs J.W Marriott Resort )
In pear, any self-pollinated progeny are difficult to produce because they have self-incompatibility. Using a self-compatible cultivar ‘Osa Nijisseiki’ with a mutation about the style phenotype, we produced self-pollinated progeny of Japanese pear (Pyrus pyrifolia Nakai) in this experiment. Twenty-eight seeds were obtained by self-pollination of S1 cultivar ‘Nou 1 gou’. Twenty-two seedlings were germinated and established in the field. All seedlings were inspected such as true S2 progenies about 42 SSR loci on a reference map of ‘Housui’ (Terakami et al, 2009). Their homozygosity (No. of locus as homozygote / No. of locus scored) estimated by the 42 loci were varied among 0.69 to 0.82 higher than 0.64 in ‘Nou 1 gou’ and 0.24 of ‘Osa Nijisseiki’. The negative correlation observed between the homozygosity and plant height in S2 individuals in first year reflected a negative effect of the homogeneity on their growth. We concluded that the S2 progeny was valuable for the genomic research such as the recessive gene effects in pear.