Somatic Embryogenesis and Histological Analysis of Zelkova schneideriana Hand.-Mazz

Thursday, July 31, 2014: 2:00 PM
Salon 8 (Rosen Plaza Hotel)
Xiaoling Jin , Central South University of Forestry and Technology, Changsha, China
Youping Sun , Hunan Academy of Forestry, changsha, China
Xijun Hu , College of Landscape Architecture, Central South University of Forestry and Technology, Changsha, China
Donglin Zhang , University of Georgia, Athens, GA
Audio File Recorded Presentation
Zelkova schneideriana Hand.-Mazz. is a high-value timber plant and popular landscape species in China. It is an endangered species due to over-harvesting and inefficient propagation systems. Immature zygotic embryos from a 20-year-old tree were cultured on Murashige and Skoog's (MS) medium containing 2.0 mg·L-1 6-benzylaminopurine (BA), and 51% of explants induced embryogenic calluses. The white friable nodular callus was sub-cultured on MS medium supplemented with 1.0 mg·L-1 BA and 1.0 mg·L-1 α-naphthaleneacetic acid (NAA) to regenerate somatic embryos and the conversion rate was 36.7%. Somatic embryos successfully germinated on MS medium without plant growth regulators. Histological analysis revealed that somatic embryogenesis of Z. schneideriana included single embryogenetic cell, multicellular proembryo, globular, heart and cotyledonary embryo. These results were similar to other Ulmaceae species. Somatic embryogenesis could be used for production of synthetic seeds and regeneration of transgenic plants.
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