Estimation of Relative Abundance of 16S rDNA of Candidatus Liberibacter asiaticus by Comparison to Host Cytochrome Oxidase DNA in Orange Juice

Monday, July 28, 2014: 2:10 PM
Salon 8 (Rosen Plaza Hotel)
Jinhe Bai , USDA-ARS, USHRL, Fort Pierce, FL
Elizabeth Baldwin , USDA, ARS, USHRL, Fort Pierce, FL
Wei Zhao , USDA, ARS, USHRL, Ft. Pierce, FL
Audio File Recorded Presentation
A protocol was developed to isolate DNA from orange juice and detect titer of Candidatus Liberibacter asiaticus (CLas), the presumed pathogen associated with citrus Huanglongbing (HLB) disease by real-time PCR. To obtain quality DNA, new methods were used to remove pectin and unidentified inhibitors.

See more of: Workshop: USDA-ARS: Highlights on Citrus Research in Florida *CEU Approved
See more of: Workshop