Search and Access Archived Conference Presentations

2014 ASHS Annual Conference

19025:
Identification of QTL Underlying Powdery Mildew and Bacterial Canker Infection in Sweet Cherry (Prunus avium L.)

Tuesday, July 29, 2014: 10:45 AM
Salon 8 (Rosen Plaza Hotel)
Yunyang Zhao, Department of Horticulture and Landscape Architecture, Washington State University, Prosser, WA
Josephine Udodirim Mgbechi-Ezeri, Washington State University, Prosser, WA
Kenneth B. Johnson, Oregon State University, Corvallis, OR
Umesh Rosyara, Horticulture, Michigan State University, East Lansing, MI
Amy F. Iezzoni, Michigan State University, East Lansing, MI
Cameron Peace, Washington State University, Pullman, WA
Amit Dhingra, Department of Horticulture, Washington State University, Pullman, WA
Nnadozie Oraguzie, Washington State University, Prosser, WA
Audio File Recorded Presentation
Powdery mildew (PM), caused by Podosphaera clandestina, and bacterial canker (BC), caused by Pseudomonas syringae pv. Syringae, are the major diseases of sweet cherry in the USA. Incorporation of natural resistance into elite cultivars would be an effective way to reduce reliance on fungicide and pesticide use and facilitate the transition to sustainable production systems. This study was designed to identify quantitative trait loci (QTL) underlying PM and BC infection to facilitate development of new resistant cultivars. Six hundred pedigree-linked germplasm were used in this study. PM was scored in the field on a 0-5 scale (0 = no visible symptoms and 5 = very severe infection on leaves) from 2011 to 2013. BC phenotyping was performed by inoculating five healthy and newly emerging leaves with 10 µl of 108 CFU/ml bacteria suspension mixed with 0.5% surfactant by mid-rib wound method in a detached leaf assay. Disease was scored in the lab on a 0-4 scale (0 = no necrosis and 4 = total necrosis). Approximately 1100 single nucleotide polymorphism (SNP) and four simple sequence repeat (SSR) markers were used for determining genome-wide marker-locus-trait associations. One PM QTL mapped on top of linkage group (LG) 5 in the three years while others mapped on LGs 1, 3 and 6 in a single year. For BC, one major QTL was identified on top of LG 5. The co-location of QTL for both diseases on LG 5 will be explored further to develop a breeding strategy for the two diseases combined.
See more of: Fruit Breeding 2 (Oral Session)
See more of: Oral Abstracts