2014 ASHS Annual Conference

Taxonomic study in external morphology analysis was conducted to understand the intraspesific difference among three habitats(Ulleungdo, Jirisan and Odaesan) of Allium victorialis var. platyphyllum in Korea. Intraspesific variations among Allium victorialis var. platyphyllum populations including three habitats and 41 individuals were investigated based on the randomly amplified DNA polymorphisms derived from PCR(polymerase chain reaction) method. External morphology such as bulb color, leaf blade length and width, petiole length, total leaf length, peduncle length, perianth lobe length and width, length of anther and filament were useful characters for the identification of populations in three habitats. Eleven primers out of 81 primers(80 random[10-mer] primers, one 15 mer[M13 core sequence] primer) screened resulted in DNA polymorphisms(61.5%) in all of the tested plants, producing 83 randomly and specifically amplified DNA fragments. Ulleungdo population was discrete from the other two populations at the 0.77 of similarity index value of DNA polymorphisms. But Jirisan and Odaesan population were not differentiated from each other. For the variation of each population, Ulleungdo population showed lower level of variation with similarity index of 0.92 than Jirisan and Odaesan populations with 0.86 and 0.87, respectively.