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Preliminary Studies on Obtaining Haploid Plants in Spinach

Tuesday, August 4, 2015: 4:15 PM
Oak Alley (Sheraton Hotel New Orleans)
Ceren Eksi , Alata Horticultural Research Institute, Erdemli-MERSIN, Turkey
Davut Keles , Alata Horticultural Research Institute 33740, Erdemli-MERSIN, Turkey
Hasan Pinar , Department of Horticulture, Faculty of Agriculture, Erciyes University 38039, Kayseri, Turkey
Atilla ATA , Alata Horticultural Research Institute 33740, Erdemli-MERSIN, Turkey
Nihal Denli , Alata Horticultural Research Institute, Erdemli-MERSIN, Turkey
Namik Kemal Yucel , University of Cukurova Faculty of Agriculture, Adana, Turkey
Hatira Taskin , University of Cukurova Faculty of Agriculture, Adana, Turkey
Saadet Buyukalaca , Department of Horticulture, Faculty of Agriculture, Cukurova University 01330, Adana, Turkey
This presented study contains the preliminary results of the project numbered as “TAGEM/BBAD/12/A09/P10/01” carried out by Republic of Turkey Ministry of Food, Agriculture and Livestock to develop protocol for obatining haploid embryo in spinach.  Pollination with irradiated pollen and anther culture methods were used to obtain haploid plants in this study. For the study of anther culture, three spinach varieties (Koto, Favorit, and Greenstar) and 4 different growth media (MS+2 mg·L-1 NAA+0.5 mg·L-1 GA3, MS+4 mg·L-1 NAA+0.5 mg·L-1 GA3, MS+ mg·L-1 NAA+1 mg·L-1 BAP+ MS+4 mg·L-1 NAA+1 mg·L-1 BAP) were tested to obtain haploid embryos. Gynogenesis studies using irradiated pollen was performed with again same three spinach varieties and three gamma ray doses (100, 150, and 200 Gy) coming from Co60. The MS nutrient medium containing 1 mg·L-1 IAA was used for embryo germination. Outcame of this study showed that different varieties produce different results on the doses of gamma ray. Embryo germination and plant regeneration from these embryos were provided from 100, 150 Gy gamma ray doses of Koto and Greenstar varieties and 150, 200 Gy of Favorit variety. Molecular studies revealed that plants obtained from the embryos were spontaneous dihaploid. In terms of both the number of embryos and the number of plants obtained, Koto variety was found to be the most successful variety and 150 Gy most successful dose.
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