TILLING and ecoTILLING for Variation in Genes for Ornamental Traits

Genomic resources and tools enable the discovery of variation in genes for horticultural traits. Candidate genes have been identified that play similar roles in the control of flowering, architecture, and disease-resistance in different crop species. Natural or induced variation in candidate genes can be detected by mutation screening techniques such as high-resolution melting (HRM) analysis. We have used HRM to screen for EMS-induced mutations in the Mlo gene of petunia. Mlo mutation could potentially lead to resistance to powdery mildew.  The USDA blueberry germplasm collection was screened for natural variation in TFL1, a gene that regulates floral initiation. Novel alleles of these candidate genes can be used for trait development in ornamental plants.