2017 ASHS Annual Conference

Gallic acid is abundantly present in fruits, and exert many biological activities such as antioxidant, anticancer, anti-aging, and whitening activities. However, the cosmetic application of gallic acid is limited due to its low bioavailability drawback. In this study, gallic acid was enzymatically transglycosylated via the acceptor reaction of a dextransucrase with gallic acid and sucrose. Gallic acid glucoside was enzymatically synthesized via the acceptor reaction of a glucansucrase from Leuconostoc mesenteroides with gallic acid and sucrose. The glucoside was purified by preparative C18 reverse-phase high-performance liquid chromatography after removing unreacted gallic acid and sucrose using butanol and water (1:1, v/v). Structure of gallic acid glucoside was identified by MALDI-TOF MS. Three variables, conc. of sucrose, dextransucrase, and gallic acid were used to determine the optimum producing condition of gallic acid glucoside with a five level central composite design and response surface methodology. Production yield of gallic acid glucoside was 114 mM by response surface methodology with a reaction mixture of 319 mM gallic acid, 355 mM sucrose, and 0.93 U/mL dextransucrase. Purified gallic acid glucoside displayed lower diphenylpicrylhydrazyl scavenging activity but a 31% increased anti-lipid peroxidation compared to those of gallic acid. However, its water solubility was increased significantly by 180% than gallic acid.

 * This work was supported by Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (NRF-2016R1D1A1B03936148).