2017 ASHS Annual Conference

In apple trees, the multiple infection of viruses and a viroid causes farmer’s economic losses, such as yield and quality decrease of fruits. Therefore, we analyzed the commercial nursery trees of main apple cultivars to confirm the infection degree with RT-PCR technique. The RT-PCR analysis for each nursery tree performed dividedly into scion and root zone. For apple cultivars of ‘Hongro’ lines, the scion analysis resulted that both ACLSV and ASGV were infected by 100%. ASPV was infected by 81.3%. ApMV and ASSVd showed no amplification bands on the all samples. Whereas, the root analysis showed that infection ratio of ACLSV, ASGV, and ASPV were 87.5, 100, and 81.3% as well as that of ApMV and ASSVd were 12.5 and 6.3%, respectively. For apple cultivars of ‘Fuji’ lines, the scion analysis resulted that each infection ratio of ACLSV, ASGV, and ASPV were 86.7, 100, and 86.7%, showing no infection of both ApMV and ASSVd. On the other hand, the root analysis showed that ACLSV, ASGV, ASPV, ApMV, and ASSVd were infected by 86.7, 93.3, 93.3, 6.7, 0%, respectively. In conclusion, our results indicate that most of commercial apple nursery trees was multiple-infected by at least 2~3 viruses, such as ACLSV, ASGV, ASPV.

Additional key words: ACLSV, ASGV, ASPV, ApMV, ASSVd