2017 ASHS Annual Conference

Glucosinolates profiles are variable depending on Brassica species. We conducted individual glucosinolates and amino acids analysis to understand the variations of glucosinolates contents and components by Brassica species. The seeds of chinese cabbage, broccoli, mustard, baemoochae, radish, and arabidopsis were plant materials. Twenty one individual glucosinolates and 21 individual amino acids were detected by LC-MS. Additionally total amino acid was determined by photometric ninhydrin method. Using these multivariable components, we analyzed statistics including PLS-DA, Heat map, Pearson’s correlation. In PLS-DA, 6 groups were separated according to component 1 (23.1%) and 2 (28.6%) and dependent on species variety. By Pearson’s correlation and heat-map analysis, total and major glucosinolate content in each species had positively correlated with total amino acid content. The content of total amino acid using photometric ninhydrin method also had a positive correlation to total and major glucosinolate contents. However major glucosinolate and their precursor amino acid had no correlation, significantly. Thus, a rapid ninhydrin method would be useful to screen higher content of glucosinolate in Brassica seeds.