2017 ASHS Annual Conference

Shoots were regenerated from in vitro leaves of a primocane red raspberry cultivar (Rubus idaeus ‘Polana’). In vitro regeneration was achieved when leaf segments and petioles of in vitro plants were cultured on woody plant medium (WPM) supplemented with 6-benzylaminopurine (BA) with or without thidiazuron (TDZ). Effects of BA and TDZ at deferent levels on shoot regeneration rate and shoot number were investigated. The maximum shoot regeneration rate from leaf segments occurred on WPM containing 2.5mM BA and 0.1mM TDZ with an average of 62.2% of explants forming shoots, while the greatest regeneration rate (41.7%) was observed on WPM containing 2.5mM BA and 0.5mM TDZ when petioles were used. An average of 3.2 and 4.7 shoots were developed from each of responsive leaf and petiole explants, respectively, in the medium with 5.0mM BA and 0.1mM TDZ. A significant number of vitrified shoots were observed in the treatments where TDZ was above 0.5mM. Regenerated shoots were transferred to Murashige and Skoog (MS) medium supplemented with 1-10 mM IBA for in vitro rooting. Rooted plants were then transferred to potting medium and grown in the greenhouse. This research will facilitate raspberry improvement using biotechnology such as in vitro mutation and genetic transformation.