2018 ASHS Annual Conference

Plants were regenerated from in vitro tissues of red raspberry cultivar ‘Joan J’. The effects of plant growth regulator (PGR), explant type, and explant age on shoot regeneration were investigated. In vitro leaf segments and petioles were inoculated in woody plant medium (WPM) with 200 mg/l polyvinylpyrrolidone (PVP) and various combinations of 6-Benzylaminopurine (BA) and/or thidiazuron (TDZ). Results showed that the highest regeneration rates were 70% and 38% when the 7-day-old leaf segment and petiole were inoculated on WPM with 2.5 µM BA and 1.0 µM TDZ, respectively. The explant at the age of 7-day-old showed higher ability to shoot regeneration than that of the 14 or 28-day-old explants. Regenerated shoots are being rooted both in vitro and ex vitro. The in vitro rooting will be carried out in 1/2 Murashige and Skoog (MS) medium with 5 µM 1-naphthaleneacetic acid (NAA), 10 µM NAA, 5 µM indole-3-butyric acid (IBA), 10 µM IBA, and the no-auxin control. The ex vitro rooting will be conducted by dipping cuttings in a solution of 500 µM NAA, 500 µM IBA or water, then inserted to potting mix. The rooting percentage, the root number, root length per responsive cutting, survival rate in greenhouse will be determined.