2018 ASHS Annual Conference
Adventitious Shoot Regeneration from Leaf Explants of Peach Rootstock ‘Hansen 536’ ([P. davidiana x P. persica] x P. persica 'Okinawa') x P. Amygdalus)
Adventitious Shoot Regeneration from Leaf Explants of Peach Rootstock ‘Hansen 536’ ([P. davidiana x P. persica] x P. persica 'Okinawa') x P. Amygdalus)
Wednesday, August 1, 2018
International Ballroom East/Center (Washington Hilton)
‘Hansen 536’ is an important commercial rootstock for peach and almond. However, susceptibility to wet soil and bacterial canker are the main disadvantages of this cultivar. Genetic engineering techniques offer an attractive approach to efficiently improve the problems existing in this cultivar. In this study, an efficient protocol for organogenesis regeneration from leaf explants were developed. Leaf explants were cultured on Murashige and Skoog (MS) and Woody Plant Medium (WPM) supplemented with different plant growth regulators to induce shoot regeneration. Nine potential media were evaluated and adventitious shoot regeneration occurred on five media at frequencies ranging from 0-34.5%. The optimal regeneration at a frequency of 34.5% occurred on the WPM medium containing 8.88 µM 6-benzylamino-purine (BA) and 1.97µM indole-3-butyric acid (IBA). The regenerated shoots were rooted successfully and transplanted to soil. This study provided a prerequisite for developing an efficient genetic transformation system.