2018 ASHS Annual Conference

Citrus junos is a universally consumed fruit in Eastern Asian countries such as Korea, China and Japan and the fruit is originally from those countries. In Korea, Jeollanamdo, one of provinces, about 81.6% of citrus junos fruits are produced per year. The fruit has very strong sour taste, so it makes hard for consumers to eat raw. Thus, consumers generally make the fruit tea with sweeteners. Citrus junos is known to containe significant amount of antioxidants including vitamins and phenolic compounds but only limited information is available how well those antioxidants are extracted when it is consumed as tea and what the best extraction condition is. The objectives of this study were to determine optimize extraction conditions and to quantified active compounds as flavonoids content (naringin and hesperidin) in extracted citrus junos oleoresin for application of food and cosmetic ingredients in the future. Citrus junos skins were mixed with several different ratios (Wt/Vol %) ethanol (>95%) for determination of yield for extraction. The best extract yield was 1:10 ratio (Wt/Vol %). For factors of temperature and time for extraction, the best conditions of temperature and time were at 40℃ and 5 hours which yield was 10.8% for extraction of oleoresin. Also other results of activity compounds of the fruit were as: 88.2 Vitamin C eq. mg/100g for antioxidant activity, 518.2mg/100g for total flavonoids [hesperidin (107.3mg/10g), narirutine (80.8mg/100g), neohesperidin (58.5mg/100g), and naringin (58.1mg/100g)]. Utilizing the results of this study, Citrus junos is higher application areas for food ingredients for producing functional drinks and snacks and cosmetics ingredients due to high content of flavonoids of the fruit.