2018 ASHS Annual Conference

Nandina domesticaThunb. (Berberidaceae) is an evergreen ornamental shrub with compact habit, colorful foliage, and wide adaptation to the landscapes and gardens in the southern US. Three newly selected clones with red, yellow and apricot foliage were trialed and desperately needed to be rapidly propagated for the market. Micropropagation was adapted and the youngest new growing tips with a naked bud were harvested as the explant. Contamination and bacteria invasion limited the 3^rdor earlier newly sprouted nodes as additional plant materials. Both red and apricot clones preferred 1/2MS basal salt while yellow one performed better under B5 without any vitrification. Both hormones, 1.0 mg/L benzylaminopurine (BA) and 2.0 mg/L gibberellic acid (GA₃), were added to the initiation media. For multiplication stage, 1/2MS basal salt with hidiazuron (TDZ) at 0.05 mg/L helped red and apricot clones to reach the average multiplication rate of 7.7 in five weeks. For yellow clone, B5 with 0.03 mg/L TDZ yielded the highest rate of 3.0. Shoot length and number of shoots per cluster also significant regulated the proliferation. Shoots of 2.5-3.0 cm long worked better for multiplication instead of 1.0 cm, 2.0 cm and >3 cm. Two shoots per cluster could significantly improve the proliferation. Shoots longer than 3 cm didn’t proliferated well but could reach 100% rooting within one month on B5 medium plus 2.0 mg/L Indole-3-butyric acid (IBA). Newly selected clones of Nandina domestica could be regenerated by micropropagation and the multiplication rate was 3 or higher.