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2019 ASHS Annual Conference

Isolation and Functional Characterization of the PHT1 Gene Encoding a High Affinity Phosphate Transporter from Camellia Oleifera

Thursday, July 25, 2019
Cohiba 5-11 (Tropicana Las Vegas)
Junqin Zhou, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan Province, 410001, China, Changsha, China
Mengqi Lu, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan Province, 410001, China, Changsha, China
Chenhui Zhang, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan Province, 410001, China, Changsha, China
Xinjing Qu, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan Province, 410001, China, Changsha, China
Jin Yang, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan Province, 410001, China, Changsha, China
Jun Yuan, Central South University of Forestry and Technology, Changsha, China
Plant phosphate transporters mediate phosphate acquisition, translocation, and recycling in plants. In this study, a putative protein high affinity phosphate transporter (GenBank KF989483.1) was isolated and characterized from tea tree (Camellia oleifera Abel.). The CoPht1;3 contained an 1575-bp open reading frame, encoding 525 amino acids residues. It shared 88.5, 86.5, and 85.6% amino acid identities with the Pht1s of Camellias sativa, Arabidopsis thaliana, and Hevea brasiliensis, respectively. The CoPht1;3 exhibited all essential motifs and 12 transmembran helix common to the Pht1 family of phosphate transporters, which α-helical structure accounted for 48.66%. The CoPht1;3 was localized in tonoplast by transient expression of CoPht1;3:eGFP in tobacco epidermal cells. Quantitative real-time PCR (qRT-PCR) analysis showed that CoPht1;3 mRNA was detected in root, leaves and stem, but its transcript was the most abundant in young leaves at low P supply. The results indicated that CoPht1;3 in response to Pi starvation might be a high Pi affinity co-transporter of H+/Pi. CoPht1;3 overexpression enhanced aborption of phosphorus and increased biomass of the transgenics. This study suggested that CoPht1;3 may play a key role in transportation process of phosphorus in oil tree leaves and could be further targeted as a candidate gene to improve tree P efficiency.
See more of: Plant Nutrient Management 2 (Poster)
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