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2019 ASHS Annual Conference

Overexpression of Recombinant Nucleoside Diphosphate Kinase 2 Gene Enhances Tolerance to Various Environmental Stresses in Transgenic Gardening Plants

Tuesday, July 23, 2019
Cohiba 5-11 (Tropicana Las Vegas)
Hye Ryun An, National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju, Korea, Republic of (South)
Nan-Sun Kim, National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju-gun, Korea, Republic of (South)
Pue Hee Park, National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju, Korea, Republic of (South)
Pil Man Park, National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju, Korea, Republic of (South)
Su-Young Lee, National Institute of Horticultural & Herbal Science, Rural Development Administration, Korea, Republic of (South)
Nucleoside diphosphate kinase 2 (NDPK2) is known to regulate the expression of antioxidant genes in plants. In this study, transgenic gardening plants expressing the recombinant NDPK2 gene under the control of an oxidative stress-inducible peroxidase (SWPA2) promoter were developed and enhanced tolerance to various abiotic stress conditions. Integration of the NDPK2 gene into transgenic plant’s chromosomes was confirmed by genomic DNA polymerase chain reaction. The level of NDPK2 mRNA expression and protein in transgenic plants following methyl viologen (MV) treatment was positively correlated with the plant’s tolerance to MV-mediated oxidative stress. We observed that transgenic plants expressing the NDPK2 showed enhanced tolerance to cold, high salinity, and drought stresses.

Keywords: Nucleoside diphosphate kinase 2, Stress-inducible peroxidase promoter, Transgenic gardening plants, Oxidative stress

See more of: Plant Biotechnology 1 (Poster)
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