2019 ASHS Annual Conference

Common bean (Phaseolus vulgaris L.) is one of the most important edible legume crops worldwide. The objective of this research was to conduct genetic diversity and population structure analysis of a world-wide collection of common bean accessions using single nucleotide polymorphism (SNPs) markers. Whole genome resequencing (WGR) was used to discover SNPs in a panel of 355 USDA GRIN common bean germplasm accessions originally collected from 46 countries. The WGR was done with 10× coverage (about 7 Gb sequencing data each accession). A total of 24.4 M SNPs among the 355 common bean accessions were identified on 11 chromosomes ranged from 1.47 M SNPs on chromosome 6 to 2.93 M SNPs on chromosome 8. The model-based program STRUCTURE 2 was used to infer population structure. Genetic diversity was assessed and the phylogeny trees were drawn using MEGA 7 based on the Maximum Likelihood tree method. The analysis resulted in a phylogentic tree with two clusters reliably separating all 355 accessions. The genetic diversity and population structures were associated with geographic collections. These data could provide genetic diversity information for breeders to select and use these accessions as parents in common bean breeding programs.