2019 ASHS Annual Conference

In this work, shoot tips from adventitious buds induced by tissue cultured bulb-scale segments were successfully cryopreserved by a droplet-vitrification method. The highest rate of shoot formation was obtained from shoot tips treated with LD1 for 60 min and PVS3 for 240 min at 23℃. A total of 160 accessions of lily as well as some Lilium species have been cryopreserved with a mean regeneration percentage of 56.7% after cryostorage. Among Lilium germplasms cryopreserved, there’re several Korean, Chinese, and Taiwanese seed stocks which have good qualities for inter-species hybrid. In this study, morphological and genetic stability of regenerated lily plants from shoot tips cryopreserved in liquid nitrogen were also examined. Ten cryopreserved lily germplasm accessions were used to assess morphological and genetic stability. No differences in morphological characteristics including flower, stigma and pollen color, spots on flowers, flower’s direction etc. between controls(fresh control and non-cryopreserved) and cryopreserved plantlets. The genetic stability of regenerants (before and after cryopreservation) was investigated using inter simple sequence repeat (ISSR) and AFLP markers. In this study, these results show that droplet-vitrification freezing cryopreservation is a practical method for long-term storage of apple germplasms.

Additional key words: lily, cryopreservation, shoot tip, droplet-vitrification, genetic stability