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2019 ASHS Annual Conference

Development of Multiplex PCR for Diagnosis of Five Potexviruses in Cactus Plant

Wednesday, July 24, 2019
Cohiba 5-11 (Tropicana Las Vegas)
Eun Gyeong Song, Ph.D., Seoul Women's University, Seoul, Korea, Republic of (South)
Chung Hwa Park, MS, Seoul Women's University, Seoul, Korea, Republic of (South)
Yoon Hyun Bang, MS, Seoul Women's University, Seoul, Korea, Republic of (South)
Hae Min Lee, MS, Seoul Women's University, Seoul, Korea, Republic of (South)
Ki Hyun Ryu, Ph.D., Seoul Women's University, Seoul, Korea, Republic of (South)
Five potexviruses, namely cactus virus X, opuntia virus X, pitaya virus X, schlumbergera virus X, and zygocactus virus X, have been reported in cactus plants. Here, a multiplex PCR based on specific DPO primers was developed to simultaneously detect these five viruses and an internal control in 18 field samples consisting of 16 cactus species. Viruses were detected in nine of the 18 cactus samples. Cactus virus X, pitaya virus X, schlumbergera virus X, and zygocactus virus X were simultaneously detected in Aporocactus flagelliformis and Notocactus leninghausii f. cristatus. Our multiplex PCR was applied successfully in the field samples and it could be useful in diagnosis of virus infection in cactus plants.
See more of: Plant Biotechnology 2 (Poster)
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