2019 ASHS Annual Conference

Berry drop of grapes is a ubiquitous phenomenon during storage and transportation. If the berry falls off the stem, the value as a product drops and the juice leaking from the falling part causes additional infection and increases the disposal rate. In the experiment, the Fruit detachment force(FDF) between stem and berry was measured using a TA-PLUS texture analyzer (Lloyd Instrument, U.S.A.). To go beyond GWAS(Genome Wide Association statistics), we exploit the panel of 263 cultivars crossed with Tano Red Χ Ruby seedless and other 14 cultivars collected from the vineyard of National Institute of Horticultural and Herbal Science(NIHHS) in Wanju. 5~10 berries with stem from each cultivar were measured. The texture analyzer was programmed to measure the force required to pull apart berry and stem. The force relates to upward movement which is perpendicular to the longitudinal axis of the stem-berry system. The mean value of the force was 1.0 to 16.6N. The set of grape cultivars underwent genotyping by sequencing (GBS), yielding 25,465 polymorphic and informative single-nucleotide polymorphisms (SNPs) after data editing. Using GBS data, cultivar phenotypes were converted into formatted data for GWAS analysis using TASSEL, GAPIT software. By TASSEL, 64 of significantly associated markers were identified from 25,465 SNPs. The P-values of the detected associations ranged from 8.12×10⁻⁸ to 8.29×10⁻¹⁶. Of these markers, the most significant associations were present on chromosome 18, which contained 56 significantly associated markers. Our findings will help future studies of the genetic analysis on the berry traits in grape.