2019 ASHS Annual Conference

Forty eight melon varieties were subjected for developing SNPs using genotype by sequencing (GBS). The SNP loci with missing data more than four genotypes were removed from total 8076 SNP loci detected from the GBS analyses. Subsequently, 5644 SNP loci were chosen and the average of polymorphism information contents (PIC) was 0.2. Two hundred seventy SNP loci with the highest PIC values were retained and their genotypes were used to conduct UPGMA tree for genetic relationships of 48 melon varieties using PAUP software. To distinguish closely related varieties, newly screened SNP loci were added and the SNP loci with similar genotypes were removed from 270 SNP loci. In conclusion, 192 SNP loci with high PIC values (0.373) were chosen for classification of melon varieties