2019 ASHS Annual Conference

± 2 ºC, under continuous dark or under a photoperiod of 16 h light and 8 h dark depending on the objective. Leaves were incubated in continuous dark for three-weeks until it was transferred to light conditions. Nodal segments treated with mercuric chloride at 0.5 % concentration for 20 minutes had the highest explant survival percent (86 ± 0.07) and the lowest amount of browning. We observed the bud break was 0.57 ± 0.04 at 0.1% HgCl₂ for 20 min. Nitsch (NB) and modified NB medium supplemented with 1.0 mg/L BAP and 0.5 mg/L Kinetin supported for better responses. Callus, root induction, and a few somatic embryos were observed. The peach tissue culture is more difficult to develop a successful protocol than any other woody fruit tree was investigated. The comparison will be presented.