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2019 ASHS Annual Conference

Uses of Vip Medium and Explant Sterilants for Tissue Culture without Autoclave and Laminar Flow *CEU Approved*

In this hands-on demonstration workshop on tissue culture with sterilants, a series of activities are proposed. After a brief overview on tissue culture, three procedures (demonstrations on medium preparation, culture initiation and subculturering) will be demonstrated by the speaker and workshop attendees will have opportunities to participate. After the conclusion of activities, the workshop will be concluded with a discussion session, led by another speaker. The presenter will summarize the activities conducted and present questions to stimulate the discussion.
Thursday, July 25, 2019: 2:00 PM
Montecristo 1 (Tropicana Las Vegas)
Coordinator:
Moderator:
2:15 PM
Medium Preparation with Msvip Hot, Initiation of Culture with Sirvip, and Subculturing
Hideka Kobayashi, Kentucky State University, College of Agriculture, Food Science, and Sustainable Systems
2:35 PM
Initiation of Culture with SirVip
Hideka Kobayashi, Kentucky State University, College of Agriculture, Food Science, and Sustainable Systems
3:00 PM
PMSubculturing
Hideka Kobayashi, Kentucky State University, College of Agriculture, Food Science, and Sustainable Systems
3:15 PM
Conclusion and Discussion
Alan H Chambers, University of Florida TREC