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2019 ASHS Annual Conference

Application of CRISPR-Mediated Genome Engineering for Breeding Horticultural Crops for Disease Resistance and Quality Traits: Progress, Challenges and Future Prospects *CEU Approved*

Objectives:
  1. To obtain current knowledge and tools available for improving horticulture crops using CRISPR-mediated genome editing
  2. To overview recent advances in CRISPR-mediated genome editing in horticultural crops breeding via case studies
  3. To determine the biotechnological challenges and limitations in using genome editing in cultivar development
  4. To discuss future deployment of genome editing technology in increasing disease resistance and horticultural qualities
  5. To discuss current rules and regulations and future perspectives on using CRISPR-genome editing in developing new cultivars

Description:

This workshop will be primarily focused on the use of CRISPR genome editing in horticultural crops. It will cover the prospective, challenges, opportunities and current status and future of using this technology in diverse set of horticultural crops. There will be talks ranging from technological development (protocol development) to status and future sighting of regulatory measures that will determine the application of this technology in horticultural industry in coming days. There is an excellent list of speakers ranging from specialties in diploid to polyploid crops, homozygous to highly heterozygous and outcrossing species, from annuals to perennials and USDA Biotechnology Regulatory Services. With recent developments in this technology, there will be information on CRISPR-genome editing for wide range of horticultural crops including regulatory aspects of the technology.

At the end of the workshop, there will be a 15-minute panel discussion which will address questions from the audience and provide
networking opportunities between speakers and audience. The discussion will highlight on how can this technology be applied in wide range of horticultural crops with limited genomic information and how can new cultivars be developed and available for commercial production using CRISPR-genome editing.

Wednesday, July 24, 2019: 2:00 PM
Cohiba 1-3 (Tropicana Las Vegas)
Coordinators:
Moderators:
2:02 PM
Lipofection-Mediated DNA-Free Delivery of the Cas9/gRNA Ribonucleoprotein into Plant Cells for Genome Editing
Wusheng Liu, North Carolina State University; Neal Stewart Jr., University of Tennessee, Knoxville; Mary Rudis, University of Tennessee, Knoxville; Mathew Cheplick, University of Tennessee, Knoxville; Reginald Millwood, University of Tennessee, Knoxville; Christine Ondzighi-Assoume, Tennessee State University; Mitra Mazarei, University of Tennessee, Knoxville; Kellie Burris, University of Tennessee, Knoxville; Jonathan Chesnut, Thermo Fisher Scientific; Jian-Ping Yang, Thermo Fisher Scientific
2:45 PM
Improving the Strawberry Fruit Quality and Disease Resistance Using Genetic Engineering Technologies: Current Progress and Challenges.
Seonghee Lee, University of Florida; Cheolmin Yoo, University of Florida; Jin-Hee Kim, University of Florida; Youngjae Oh, University of Florida; Heqiang Huo, University of Florida; Vance M Whitaker, University of Florida