Identification of Biomarker Compounds In Muscadine

Muscadine phenolic compounds may have anti-oxidant and anti-inflammatory properties. In order to identify these compounds, muscadine (Vitis rotundifolia) pomace (cv.Noble) was acid hydrolyzed and extracted using solid phase extraction.  The flavonoid and anthocyanin fraction (FAF) was analyzed for phenolic and flavonoid contents using colorimetric assays, and individual biomarker contents using high performance liquid chromatography (HPLC). The FAF had greater phenolic and flavonoid contents as compared to crude extract. The main anthocyanins present were di-glucosidic delphinidins and cyanidins.  The FAF fraction was tested in two colon cancer cell lines to determine the anti-inflammatory nature of the muscadine biomarker compounds.  Preliminary studies using cell proliferation assays and flow cytometry data showed that this fraction decreased viable cell proliferation in HT-29 and HCT-15 cell lines and evidence of G1 arrest, indicating possible anti-inflammatory role of muscadine in colon cancer cells.