Search and Access Archived Conference Presentations

The 2011 ASHS Annual Conference

Variation In Antioxidant Enzyme Activities Among Blueberry Cultivars (Vaccinium species)

Monday, September 26, 2011
Kona Ballroom
Shiow Ying Wang, Ph.D., Genetic Improvement of Fruits and Vegetables Laboratory, USDA ARS, Beltsville, MD
Hangjun Chen, Food Quality Laboratory, U. S. Department of Agriculture, Beltsville, MD
Mark Ehlenfeldt, USDA–ARS, Marucci Center for Blueberry & Cranberry Research, Chatsworth, NJ
Fruit from forty-two blueberry (Vaccinium species) cultivars including thirty-six rabbiteye rabbiteye cultivars (Vaccinium ashei Reade), three V. ashei hybrid derivatives and three northern highbush (V. corymbosum L.) standards were evaluated for antioxidant activities (AA), levels of antioxidant enzymes [catalase (CAT), ascorbate peroxidase (AsA-POD), glutathione reductase (GR), glutathione peroxidase (GSH-POD), superoxide dismutase (SOD), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDAR), and guaiacol peroxidase (G-POD)], and antioxidant nonenzyme components [ascorbic acid (ASA) and glutathione (GSH)]. The results showed that cultivars had varying levels of AA, a wide range of antioxidant enzyme activities, and various amounts of nonenzyme components. The correlations between AA and all of the enzyme activities or nonenzyme components were positive and high in the forty-two cultivars tested in this study. The correlation coefficients of r values between AA and CAT, SOD, DHAR, MDAR, GSH-POD, GR, G-POD, and AsA-POD were 0.91, 0.91, 0.91, 0.91, 0.90, 0.90, 0.90, and 0.90, respectively. The correlations between AA with ASA and GSH were 0.94 and 0.92, respectively. Among all 42 cultivars, ‘Early May’ had the highest activities of the AA, AsA-POD, G-POD, DHAR, and MDAR; and the highest amounts of ASA and GSH; whereas ‘Elliott’ had highest levels of GR, GSH and SOD. The cultivars with high antioxidants and antioxidant enzyme activities could be useful in the breeding program for the development of more nutritious blueberry cultivars with improved health-promoting quality for consumers.